Metabolic state of human blastocysts measured by fluorescence lifetime imaging microscopy
M. Venturas, J. S. Shah, X. Yang, T. H. Sanchez, W. Conway, D. Sakkas, D. J. Needleman
Human Reproduction, 2022 Jan 6
A coarse-grained NADH redox model enables inference of subcellular metabolic fluxes from fluorescence lifetime imaging
Xingbo Yang, Gloria Ha, Daniel J Needleman
eLife, 10:e73808, 2021 Nov 22
Metabolic imaging of human cumulus cells reveals associations among metabolic profiles of cumulus cells, patient clinical factors, and oocyte maturity
Marta Venturas, Xingbo Yang, Kishlay Kumar, Dagan Wells, Catherine Racowsky, Daniel J. Needleman
Fertility and Sterility, 2021 Sep 2
Physical bioenergetics: Energy fluxes, budgets, and constraints in cells.
Xingbo Yang, Matthias Heinemann, Jonathon Howard, Greg Huber, Srividya Iyer-Biswas, Guillaume Le Treut, Michael Lynch, Kristi L Montooth, Daniel J Needleman, Simone Pigolotti, Jonathan Rodenfels, Pierre Ronceray, Sadasivan Shankar, Sadasivan Shankar, Sadasivan Shankar, Iman Tavassoly, Shashi Thutupalli, Denis V Titov, Jin Wang, Peter J Foster
Proceedings of the National Academy of Sciences of the United States of America, vol. 118(26), 2021 May 28
Spatial gradient sensing and chemotaxis via excitability in Dictyostelium discoideum.
Daniel P. Shams, Xingbo Yang, Pankaj Mehta, David J. Schwab
Physical Review E, vol. 101(6), 2020 May 11, pp. 62410-62410
The objective of this project is to understand the spatiotemporal dynamics of energy usage in cells, how cells partition energy into different cellular processes and the associated energetic constraints.
Tissue mechanics and morphogenesis (current)
We are developing techniques to measure and model mechanical forces in tissues and embryos. The goal is to understand how mechanical forces impact collective cell migration and morphogenesis.
Self-organization of active particles (current)
Active particles dissipate free energy to move. I am studying how global order emerges from the interactions of a group of active particles. These model systems help understand cytoskeletal remodeling, morphogenesis and animal flocking.
Mechanosensing and chemotaxis (past)
Cells can sense their environment through mechanical or chemical cues. We explored how cells sense their environment and adapt their motion.
Division-control in freshwater planarians (past)
Planarians are multicellular flatworms that are capable of asexual reproduction through fission and regeneration. We studied how planarians control their divisions over their life cycle.